Cy3 alexa

cy3 alexa Among currently available fluorescent dyes the cyanine dyes are better able to withstand the harsh dehydration and embedding conditions required for mounting sections in non polar plastic media such as DPX and Permount . Apr 26 2002 trackers e. Semrock Set Alexa Fluor 514 518 543 1 Star. 8 Jun 2018 Bringing Alexa Voice Service to your small connected device is now easier than ever thanks to X CUBE AVS our software package that ports nbsp 7 Aug 2020 Still having difficulties Watch the video below. Additionally cells were preincubated for 40 min with 500 nM MitoTracker 633 Molecular Probes . BS C 1 cells were immunostained for microtubules with Alexa 488 Cy3 Alexa 647 or Cy5. 5 576 589 568 543 Rhodamine Red X 570 590 PE Dyomics 590 488 599 For example Atto 488 is a superior alternative to fluorescein and Alexa Fluor 488 producing conjugates with more photostability and brighter fluorescence. Alexa 488 carboxylic acid succinimidyl ester Alexa 532 Cy3 and Sulfo Cy5 are also often referred to as Cy3 or Cy5 elsewhere the nomenclature is inconsistent Mar 07 2020 a Immunofluorescent double staining of PD 1 Cy3 red and PD L1 Alexa Fluor 488 green . Alexa and Bodipy dyes Cy2 Cy3 DAPI Hoechst 33342 propidium iodide FITC and rhodamine. Cy5 632 nm Alexa Fluor 488 475 nm LED 537 26 nm. Cyanine3 is a cyanine fluorophore with yellow emission. alexa s marketing stack You ll find all the tools you need to improve your SEO and PPC strategy prove your success and discover new ideas for growing your traffic. Indocarbocyanine Cy3. 5 Alexa Fluor 647 Alexa Fluor 680 DyLight 649 DyLight 680 IRDye 680. However APC tends to be brighter while Alexa Fluor 647 is more optimal for intracellular applications. 5 0. Cy3 534 nm LED 607 36 nm. 4 HC PL Apo CS Oil Leica SP8 inverted Room 225. Cy3 can be used as an alternative to Alexa Fluor 555 DY 550 Rhodamine Red X and TAMRA 5 6 Carboxytetramethylrhodamine . SNAP Surface Alexa Fluor 546 is a photostable fluorescent substrate that can be used to label SNAP tag fusion proteins in solution or on the surface of living cells. 5 598nm 581nm Cy3 Cy3 conjugates are only recommended for detection of moderate to high abundance targets. Properties The Alexa family covers a wide range of excitation and emission spectra from visible to infrared light and are classified according to their excitation maxima Table 1 . Cy5 Filter Set US 450. 7082210 Alexa Fluor 488 conjugated secondary antibodies were from Molecular Probes and Cy3 Cy5 and peroxidase conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories. Alexa 594. 5 56 Fluoro Emerald 26 67 Oregon Green 488 46 Alexa Fluor 647 Ex max 650 nm Em max 668 nm conjugates are highly photostable and remain fluorescent over a broad pH range. University of Miami Miller School of Medicine. 3 PE. The absolute activation rates were rapid for each pair at its corresponding optimal wavelength with values 10 s 1 or greater at only a few hundred W of laser power. 565 553 551 85 442 565 545 628 475 650 542 530130 70 ao 525150 780 39 60 575125 550140 610 20 450150 670 30 670130 610r20 530 30 505 505 550 535 600 635 600 CCCTAA repeats C rich leading strand Reacts to human mouse rat etc F1002 TelC Cy3 Cy3 F1003 TelC Cy5 Cy5 F1004 TelC Alexa488 Alexa Fluor 488 F1009 2 E10. 1 Alexa Fluor 594. B. The center of mass between the Cy3 binding sites and the Alexa 647 binding sites is either 10 nm 20 nm or 40 nm. 18 3. 5 and Atto 565 red absorbing Alexa Fluor 647 Cy5 Atto 647 Atto 647N Dyomics 654 Atto 655 Atto 680 and Cy5. . 1 Alexa Fluor 680. Newsletter. . 1 Alexa Fluor 555. 602 red. Often used for microscopy Cy3 channel is available on many instruments. Lifetime Data of Selected Fluorophores. 2 Alkaline Phosphatase AP Application Please inquire Alternatives 219 Western Dye Full name Excitation max Emission max DAPI 4 6 Diamidino 2 phenylindole 358 461 PI Propidium iodide 535 617 A488 Alexa 488 498 519 FITC such as Cy3 and Alexa dyes A. F1006 TelG Cy3 . Alexa Fluor 488 1 Apply Alexa Fluor 488 filter Useful for fluorophores related to rhodamine Cy3 and fluorescent proteins with similar emission spectra After incubation for 30 minutes the cells were fixed and stained with phalloidin Alexa 488 anti CD3 and anti mouse LFA 1 followed by avidin Cy3 or anti mouse IgG APC. The Alexa Fluor 633 . Cy2 and Cy5 were discontinued several years ago after the brighter DyLight and Alexa Fluor dyes were introduced however Cy3 has remained in our line up of fluorescent dye conjugates due to its brightness and photostability. The Alexa Fluor 647 dye was considerably more photostable than the Cy5 dye over the duration of the photobleaching experiments and retained about 80 of the initial fluorescence whereas the Cy5 dye retained only 55 . 5 tetramethylrhodamine TMR or Texas red were coupled to the 5 amino group. As advertised they seem to be at least a little brighter and more photostable than the Alexa 647 and Cy3 dyes we had been using. EYFP Venus Citrine graph 25 mm 540 25 565 605 55. ____ 574 nbsp Chroma Catalog Sets ET Alexa Fluor 568 Cy3. Sulfo Cyanine3 NHS ester can replace Cy3 Alexa Fluor 546 and DyLight 549. Dansyl 340 578. Laser Oct 19 2005 The dependence of developing spinal motoneuron survival on a soluble factor s from their target muscle tissue is well established both in vivo and in vitro . ATTO 465 Cy3. 545 40 . 5 NHS ester dye pre dissolved in DMSO 218 M 44 L was added. 5 633nm Alexa 633 Alexa 647 Cy5 Objectives 10x 0. Cy3 546 563 Alexa Fluor 546 Alexa nbsp Alexa fluor 546 556 573. They are the best choice for immunofluorescence detection in the far red region of Cy3 is a bright water soluble and pH insensitive orange fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC tetramethylrhodamine filter sets. All analyses were performed on more than 100 cells. 3 Jan 2019 The Cybic Legend is being unveiled at the Consumer Electronics Show CES in Las Vegas and comes equipped with Amazon 39 s Alexa digital nbsp . The majority of CD3 positive cells bear the PD 1 marker. 5 590 605 Cy3. 578. 3 A to F . Cy3 conjugates of antibodies peptides and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC tetramethylrhodamine filter sets. Atto 550 is an alternative to rhodamine dyes Cy3 and Alexa Fluor 550 offering more intense brightness and increased photostability. 570 yellow. 7082253 Alamar Blu Amplex red RFP excitation . F1002 amp F3001 CENPB FAM . Alexa fluor 546. Even Cy3 should work in that range. 5 is added for benzo fused cyanines. H7 Alexa Fluor 350 Qdot 565 at 545 Fluor 610 Indo I Blue Low Ca2 H t Red A Fluor 63 Alexa Fluor 514 406 4 . synthesis of Cy3. R phycoerythrin PE 489 565. Rhodamine Red X 560 580. This is a cell impermeable substrate based on Alexa Fluor 546 It is suitable for standard TAMRA or Cy3 filter sets Cy3 Cy2 Alexa 405 Cy5 . 8 Acridine Red Alexa Fluor 546 Alexa Fluor 555 Alizarin Red BODIPY probes Calcium Orange DiI DiIC16 DiIC18 ethidium bromide NeuroTrace 530 615 propidium iodide Pyronin B RedoxSensor Red CC 1 rhodamine derivatives Sevron Brilliant Red and TRITC Cy3 Alexa Fluor 555 Quasar 570 DY 554 BODIPY 558 568 BODIPY 564 570 BODIPY TMR X Oyster 556 DY 555 DY 556 Alexa Fluor 546 DY 547 NED Dragonfly orange ATTO 550 DY 560 Rhodamine Red X TAMRA DY 510XL BODIPY 581 591 CAL Fluor Red 590 ATTO 565 PET FAR Fuschia DY 590 ROX Alexa Fluor 568 Texas Red X CY3. Before mounting DNA was stained with 1 mol L TO PRO 3 for 15 minutes. Alexa Fluor nbsp Atto Rho6G 533 557 Alexa Fluor 532 BODIPY 530 550 CAL Fluor Orange 560 DY 530 Rhodamine 6 VIC . All the Alexa Fluor dyes are sulfonated forms of different basic fluorescent substances like fluorescein coumarin cyanine or rhodamin e. This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Alexa Fluor 594 conjugates Amax 591 nm Emax 614 nm emit in the red range of the visible light spectrum are brighter than other red fluorescing dye conjugates and allow better color separation from green fluorescent dyes like Alexa Fluor 549 Cy3 or TRITC. Browse Cyanine dye Conjugates Cy3. Fura 2 Indo 1. 5 Filter Set 49031 The 49031 is used with Alexa Fluor 568 CAL Fluor Red 590 and Cy3. This reagent is ideal for the labeling of soluble proteins peptides and oligonucleotides DNA. The spectra of Alexa Fluor 555 dye are an almost exact match to those of the Cy3 dye Figure 1. Red excitation and Cell Titer. They are available in a wide spectral range from blue to deep red. Alexa Fluor 488 EGFP AttoPhos O580 580 Cy3 Deep Purple HEX Alexa Fluor 532 and 546 SYPRO Ruby SYPRO Red EtBr Pro Q Diamond ROX R670 670 Cy5 Alexa Fluor 633 TOTO 3 DDAO Phosphate Two stages Fig 3 give the correct positioning and stability for optimal imaging. Samples that can be scanned include Other filter options available including DAPI Alexa 350 FITC Alexa 488 Cy3 Tritc Alexa 568 mCherry TxRed Alexa 594 Alexa 633 Cy5 Alexa 647 Doing Business with Experimental Histopathology Thomas Building DE 360 488 Alexa Fluor 488 Atto 520 fluorescein FITC and Cy2 yellow absorbing Cy3B Alexa Fluor 568 TAMRA Cy3 Cy3. 1. 00 The Alexa Fluor 555 dye with nearly identical spectral properties to Cy3 dye produces brighter more photostable conjugates. The relative fluorescence intensity signals RFI of the dye labeled antibody solutions were measured using a spectrofluorimeter and a HeNe laser with an excitation wavelength of 544 nm emission wavelength was optimized for each dye conjugate tested. of activated Cy3. S1 . poss vel controlar por comando de voz praticamente quase tudo desde a m sica filme nbsp Alexa built in products work with Alexa skills and Alexa compatible smart home devices bringing familiar capabilities from the Amazon Echo family of devices to a nbsp In addition v3 is required for Works with Alexa certification. Feb 04 2014 Cy3B NHS ester Sulfo Cy3 maleimide and Sulfo Cy5 maleimide were obtained from Amersham GE Healthcare Biosciences Pittsburgh PA . qxd. The Cy3 fluorophore is also the basis for the lipophilic neuronal and long term DiI cell tracing reagents which append alkyl tails 12 carbons onto the core fluorophore. Tamara 565 580. Cyanine3 NHS ester is an analog of Cy3 NHS ester and can replace Alexa Fluor 546 as well as DyLight 549. 5 and other similar fluorophores such as Alexa Fluor 680 Alexa Fluor 697. 553. Quasar 570. g Cy3 or Alexa Fluor 488 Biotin and Digoxigenin labeling suitable for preparation of both short gt 100 bp and long DNA probes up to nbsp F1002 TelC Cy3 . For example the MitoTracker Red absorption maximum of 579 nanometers almost exactly matches the corresponding mercury line whereas Cy3 maximum at 548 nanometers efficiently absorbs anti Cy3 Antibody A 6 is a high quality monoclonal anti Cy3 antibody also designated anti Cy3 antibody suitable for the detection of the anti Cy3 protein. Ideal for use with Yellow Cy3 DsRed Alexa Fluor 555 nbsp Alexa Fluor 594 and Texas. 5 Atto 680 IRdye 680 Alexa 680 amp 700 DyLight 680 NIR 740nm Cy7 Alexa 750 DyLight 750 IR 780nm IRdye 800 Alexa 790 DyLight 800 Mar 04 2020 We offer individual filters and filter sets targeted at common fluorophores BFP CFP WGFP GFP FITC Alexa Fluor 488 YFP tdTomato TRITC Texas Red mCherry and Cyanine CY3. TMR TRITC. 5 Cy5 Cy5. com or Faxed to 443 638 0394 RiboGreen Alexa 555 B Phycoerythrin Q Dot various SybrGreen SybrGold FRET White LEDrange 460 650 nm uses virtual filtering Example Applications Cy3 Alexa 555 Alexa 568 Cy5 Alexa 647 Sulforhodamine 101 5 CMTR Q Dots various TET Hex Molecular beacons NanoDrop Products Patented Retention System All NanoDrop products utilize a The values obtained for Alexa 405 Cy5 Cy2 Cy5 and Cy3 Cy5 were used for normalization at 405 nm 457 nm and 532 nm respectively. 575. Click iT Plus Alexa Fluor 647 Picolyl Azide Toolkit 1 Coenzyme A Packaged in Glass Bottle 1 Cy3 Ab Labeling Kit 1 Cy3 Bis NHS Ester 1 Cy3 Hydrazide 1 Cy3 Maleimide 1 Cy3 Maleimide Mono Reactive Dye 5 Pack 1 Cy3 Mono Reactive Dye Pack 1 Cy3 NHS Ester 1 Cy3 bis NHS Ester 1 Cy3 Streptavidin 1 Cy5 Ab Labeling Kit 1 Secondary antibodies were raised in donkey against each of these host species and were obtained from Jackson Immuno Research Laboratories West Grove PA conjugated to Alexa Fluor 405 488 649 or Cy3 and used at 1 600. 2004 was a generous gift from Naoko Morinaga Chiba University Japan . Alexa Fluor 546. Alexa fluor 568. This dye is pH insensitive over a wide molar range which ensure nbsp In the Cy3 like group the Alexa Fluor. Its absorption and emission spactra are almost identical to those of Alexa Fluor 555 CF 555 Dye or any other Cyanine5 based fluorescent dyes. 2ml 143. 600 40. No. 2. 5. All This Lipocalin 1 antibody is conjugated to Cy3 1 Alexa Fluor 488. 556. Cy2 489 506 714 QY 0. 3 Cy5. As seen in the figures DAPI and Alexa 488 exhibit different excitation and emission profiles over various wavelength DAPI is excited well with 820 840 nm laser whereas Alexa 488 BioVision s EZLabel Protein Cy3 Labeling Kit provides an easy way to label proteins with Cy3 in a user friendly spin column format. 3 Cy7. 1 Alexa Fluor 750. Alexa Fluor 532 530 555. IRIS 3 550 562 CY3 Alexa 546 Tetramethylrhodamine. Monoclonal anti mouse IgG was labeled using Atto 550 Alexa 555 or Cy3 dyes see Table 1 . 29 . Jan 01 2020 The obtained images were analyzed using ratio images Alexa 647 red Alexa 488 green channel with Image J. Biophys Chem 198 36 44. 581. 5 mm a 800 m b Cy3 NHS Ester also sold under Sulfo Cyanine3 NHS ester name is a bright water soluble and pH insensitive from pH 4 to pH 10 orange fluorescent dye. Cy 3 is brighter more photostable and gives less background than other orange red fluorescing dye conjugates. 4 494 519. They are brighter. Cyanine dyes are bright and stable fluorescent dyes Alexa Fluor Dyes. The Cy3 HYQ set is recommended when studying the following fluorophores Cy3 Cy3. Supplier of DNA RNA Fluorophores Alexa Fluor Cy3 Cy5 Dark Quenchers Fluorescein Dyes Li Cor 5 Rhodamine Dyes WellRed Texas Red Gene Expression bioWORLD Alexa Fluor 568 Cy3. Product Name Size C rich probe repeats of CCCTAA F1001 TelC FAM 5 nmole F1002 TelC Cy3 5 nmole F1003 TelC Cy5 5 nmole F1004 TelC Alexa488 We are closely monitoring the situation with COVID 19 coronavirus and following the guidance of the Centers for Disease Control and Prevention CDC and federal state and local health authorities. Ordering. Cy3 has excitation emission at 550 570 nm. Cy3. 12. 5 Filter Set US 450. Alexa Fluor 750 752 776 1300. Alexa 555. Cy7 is a near IR fluor that is invisible to the naked eye excitation emission maximum 750 776 nm . Alexa Fluor 790 782 804 1750. Properties and structures A range of Alexa dyes is available with emission wavelengths spanning the visible spectrum and extending into the infrared region of the spectrum. Excitation passbands are matched to discrete triggerable LEDs appropriate for excitation of DAPI FITC TRITC Cy5 and similar fluors. 2 569 nm 666 nm For COX anti mouse Alexa fluor 647 Molecular Probes or Cy3 Chemicon International was used. Peptide Synthesis Fitc Fam Cy3 Cy5 Alexa Fluor Atto Newer synthetic fluorophores including the MitoTrackers Cyanine Cy series and Alexa Fluor dyes have been specially tailored to match mercury spectral lines. Cy5. Cite. Alexa Fluor 532 44 CY3. ____ 568 nm SYPRO Orange. Absorbance 550nm Emission 575nm Extinction Coefficient 150 000. If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at 24 C for long term storage and for short term at 5 C. All dyes were dissolved according to the producer 39 s manual and applied on a glass slide. Cy3 is a better alternative to Alexa Fluor 555 Rhodamine Red X and TAMRA due to its high extinction coefficient and far red emission. In accord ance with the two photon excitation theory the majority of the investigated uorochromes did not reveal signi cant dis crepancies between the two photon and the one photon emis sion spectra. Alexa Fluor 532 534 553 nbsp Alexa Fluor 488 4. Cy 3 can also be paired with Alexa nbsp Alexa 546 is a yellow fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. Alexa Fluor Alexa Fluor 647 632 nm LED 710 40 nm. Capture time lapse images of each fluorescent dye Cy3 Alexa 488 and Alexa 647 in NHEK with 0. 5 632 nm LED 710 40 nm DyLight 488 475 nm LED 537 26 nm DyLight 550 534 nm LED 607 36 nm DyLight 633 632 nm LED 710 40 nm DyLight 650 632 nm LED 710 40 nm DyLight 680 680B 632 nm LED 710 40 nm Alexa Fluor 488 475 nm LED 537 26 nm Alexa Fluor 546 534 nm Newsletter. Cy Dyes. hydroxysuccinimidyl ester dyes the Alexa Fluor 555 dye was similar to the Cy3 dye and the Alexa Fluor 647 dye was similar to the Cy5 dye with respect to absorption maxima emission maxima Stokes shifts and extinction co efficients. A web application for viewing and comparing spectra of various fluorescent compounds. 5 25 FluoProbes 547H 38 NorthernLights 557 12 Alexa Fluor 546 6 39 48 58 CY5 24 Fluorescein FITC 5 22 25 27 54 60 65 Oil Red O 59 Alexa Fluor 555 15 26 41 68 CY5. bs 0432R A488 Chicken IgY Polyclonal Antibody ALEXA FLUOR 488 Conjugated size 0. 1. Anti Cy3 Cy5 polyclonal antibody Alexa Fluor dyes DyLight Fluor dyes R phycoerythrin R PE at scales from less than 100 g up to 1 g of IgG antibody. Each pair of spin columns provided in the kit can be used to purify up to 1 mg of the labeled target protein. This topic nbsp Our Wi Fi devices include the Smart Plug Smart Switch or C Reach. Alexa Fluor 488 3 FITC 2 Cy3 2 Alexa Fluor 594 1 Immunogen species. Note that the molecules we have named Sulfo Cy3 and Sulfo Cy5 are also often referred to as Cy3 or Cy5 elsewhere the nomenclature is inconsistent . ATTO 590. TRITC. This single band filter set is tailored for use with Cy3 and is also suitable for use with DsRed Alexa Fluor 555 Calcium Orange LysoTracker Yellow MitoTracker Orange Phycoerythrin Rhodamine Phalloidin and 5 TAMRA. Alexa Fluor 647 R phycoerythrin streptavidin pH 7. ____ 572 nm Alexa Fluor 546. Description Cy3 labeled BSA is a fluorescent BSA conjugate with bright red fluorescence. 5 is a near infrared IR fluorescence emitting dye excitation emission maximum 678 694 nm . Water soluble Cy3 is also available. Within groups roughly in order of excitation wavelength families excepted . 1 Recommendation. com 1 844 718 3550 Toll Free 1 805 504 2220 general inquires 1 805 277 0629 Fax 1 805 277 0227 tech support Cy3 dye and are readily excited by the strong 546 nm emission of mercury arc lamps and the 543 nm spectral line of the He Ne laser. 1 Alexa Fluor 647. FluoTag X2 anti ALFA is derived from an in house developed single domain antibody sdAb recognizing the ALFA tag with high affinity and specificity. Cy3 NHS Ester also sold under Sulfo Cyanine3 NHS ester name is a bright water soluble and pH insensitive from pH 4 to pH 10 orange fluorescent dye. b Immunofluorescent double staining of PD 1 Cy3 red and CD3 Alexa Fluor 488 green . 3. The STORM image shows a drastic improvement in the resolution of the microtubule network as compared to the conventional fluorescence image Fig. C. We demonstrate that although endogenous expression of heat Cy3 Filter Set US 450. Bioz Stars score 89 100 based on 26 PubMed citations. 5 581. Join for exclusive promotions amp news Submit. Keyword Research Competitive Click iT Plus Alexa Fluor 647 Picolyl Azide Toolkit 1 Cy3 Ab Labeling Kit 1 Cy3 Bis NHS Ester 1 Cy3 Hydrazide 1 Cy3 Maleimide 1 Cy3 Maleimide Mono Reactive Dye 5 Pack 1 Cy3 Mono Reactive Dye Pack 1 Cy3 NHS Ester 1 Cy3 bis NHS Ester 1 Cy3 Streptavidin 1 Cy5 Ab Labeling Kit 1 Cy5 Bis NHS Ester 1 Cy5 Hydrazide 1 Cy5 Although Alexa Fluor 488 is a fluorescein derivate in contrast to FITC it has a better stability brightness and lower pH sensitivity. The excitation and emission maxima are nearly identical to those of APC. Posts about Cy3 written by Stratech_Scientific. F3006 Cent FAM F1003 TelC Cy5 Cy5. TRITC Cy3 TagRFP Alexa Fluor nbsp Alexa Fluor 700 696 719 1400. This is in contrast to Cy2 and Cy5 which are outperformed in aqueous media by Alexa Fluor conjugates. Dy 549. Alexa Fluor 546 Alexa Fluor 633 . Abdulreda. 5 Cy7 and Cy7. 00. red Automatic detection of red fluorescence using dyes such as Cy5 Cy5. 346. Alexa Fluor 555 Dye A Superior Alternative to the Cy3 Dye Spectra of the Alexa Fluor 555 conjugates virtually match those of the Cy3 dye resulting in an optimal match to lters designed for that dye. Jun 04 2008 Immunolabeling was visualized with a fluorescence microscope and images were collected with a Leica DM confocal scanning microscope using a 10 objective with the channels for Cy3 red and Alexa 488 green used sequentially. Cy2 which is an oxazole derivative rather than indolenin is an exception from this rule. Jul 26 2019 Cyanine dyes cy2 cy3 and cy5 confocal microscopy fluorops for real time pcr dyes cyanine dyes. 596 red. Both Cy3 and Cy5 fluorescence spectra are recorded independently by the intensified charge coupled device. What Researchers are Saying about Janelia Fluor Conjugates quot We have been using the JF secondary antibodies and like them a lot for IHC. Cy3 conjugated Shiga toxin 1 Stx1 Miyake et al. Atto 488 is a superior alternative to fluorescein and Alexa Fluor 488 producing conjugates with more photostability and brighter fluorescence. Properties For facs or microscopy Alexa 1 conjugate. ATTO 647N. 10_MicroscopyTools 632 645. Cyanine Dye 3 Cy3 has a strong absorption peak at 552nm. The suffix . 5 filter Cy3 3 Quad band catalog set for Multi LED light engines like those manufactured by Lumencor CoolLED Excelitas and Cairn Research. 1 m Bates Huang Dempsey and Zhuang Science 2007 Cy3 Alexa 647 Clathrin Cy2 Alexa 647 Microtubule Crosstalk subtracted order pnabio. APC tends to be brighter while Alexa Fluor 647 is more optimal for intracellular applications. 0 and then 400 equiv. 5 bovine serum albumin at room temperature for 1 h with primary antibodies and subsequently incubated for 60 min with secondary antibodies labeled with CY3 or Alexa 488 Jackson Laboratories West Glove PA . Published Articles PNA FISH probe Cat. 7 of the Cy3 A 42 area occupying CD13 pericyte profiles respectively Alexa Fluor 546 5 Apply Alexa Fluor 546 filter Alexa Fluor 555 4 Apply Alexa Fluor 555 filter Alexa Fluor 568 1 Apply Alexa Fluor 568 filter AsRed2 2 Apply AsRed2 filter Atto 550 5 Apply Atto 550 filter CAL Fluor Red 590 1 Apply CAL Fluor Red 590 filter Cy3. Similar differences were observed when the other cyanines Cy3 and Cy5 were compared with corresponding Alexa Fluor and DyLight dyes. Alexa Fluor 647 Dyes Alexa Fluor 750 Dyes CFDA Dyes Cy3 Dyes Cy5 Dyes Cy7 Dyes Dihydroethidium Dihydrorhodamine 123 DyLight 488 Dyes DyLight 550 Dyes For double labeling Cy3 has been typically used with Cy5 with fluorescein using narrow band pass emission filter to minimize Cy3 fluorescence in the FITC filter set and also with with Alexa Fluor 647 for multiple labeling when using a confocal microscope. For double labeling Cy3 has been typically used with Cy5 with fluorescein using narrow band pass emission filter to minimize Cy3 fluorescence in the FITC filter set and also with with Alexa Fluor 647 for multiple labeling when using a confocal microscope. anti Cy3 Antibody A 6 is available as both the non conjugated anti anti Cy3 antibody form as well as multiple conjugated forms of anti anti Cy3 antibody including agarose HRP PE FITC and multiple Alexa Fluor conjugates. 5 x105 cm 1 M 1. 1 Table of Fluorochromes. 442. 5 can replace sulfoRhodamine 101. Rhod 2 Alexa Fluor 546 and CY3 emission. For detection of low abundance biological targets we recommend using AF 555 Maleimide Alexa Fluor 555 analog which can attached to proteins at high molar ratios without significant self quenching enabling brighter conjugates and more sensitive detection. P 7 19 07 5 50 PM Page 640. 00 This filter set is ideal for use with Cy5. These conjugates are ideal for nbsp Cy3. using dyes such as Cy3 Flamingo Krypton Pro Q Diamond Alexa Fluor 546 DyLight 549 Rhodamine. 00 This is a single band filter set tailored for used with Cy5. 573 yellow Cy3. now part of Life Technologies Inc. 6th Sep 2012. 2000 Shin et al. 4 554 570 CY3. The Cy5 HYQ filter combination is designed to optimize detection of fluorescence from the fluorochrome Cy5 and the set also provides excellent performance for the majority of fluorophores excited by red wavelengths. It has previously been reported that the classical fluorescent dyes used for microarray technology Cy3 and Cy5 are not ideal due to the decreased stability and fluorescence intensity of the Cy5 dye relative to the Cy3 such that dye bias is an accepted phenomena necessitating dye swap experimental protocols and analysis of differential dye The cells were then incubated in CMF PBS containing 0. 3 HC PL Apo CS2 Oil 63x 1. The rate of conversion back to the fluorescent species depends on the proximity of the secondary effector Cy3 . 29 Cyanine 3. Rox. They have a longer lifetime and bleach more slowly. 7 HC PL Apo CS Air 40x 1. The white arrow indicates representative actin cloud. Photoirradiate cells for 40 s with a 405 nm laser attached to the microscope. Alexa Dyes have high quantum yield and are relatively photostabile. 9 and therefore optical Note that Cy3 conjugates are bright and photostable in the aqueous environment as well as in non polar media. Due to nearly identical excitation and emission properties APC and Alexa Fluor 647 cannot be used simultaneously. 3 Alexa Fluor 350. In addition the Fluorophores tab provides information on the alternative fluorophores suitable for these filters. Mar 06 2019 Alexa dyes are frequently used as labels in fluorescence microscopy flow cytometry and other fluorescence based applications. 7082224. PD 1 and PD L1 are expressed poles apart from each other. 603 red. 550. Human 13053 Mouse 153 Rabbit 6 Rat 2 with 10 Trehalose. for both fluorescent e. Cy3 512 550 570 nbsp 19 Aug 2005 The red orange fluorescent Alexa. Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. 5 . Cy2 Cy3 and Cy5 Conjugates for Permanent Mounting. Alexa 633. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Cy 3 is an orange fluorophore commonly used in applications such as immunolabeling nucleic acid labeling fluorescence microscopy nbsp As N hydroxysuccinimidyl ester dyes the Alexa Fluor 555 dye was similar to the Cy3 dye and the Alexa Fluor 647 dye was similar to the Cy5 dye with respect to nbsp ____ 565 nm Cy3. g. 1 Zebrafish 1 Staphylococcus aureus 1 Medicago truncatula 1 pH 7. Cy stands for 39 cyanine 39 and the first digit identifies the number of carbon atoms between the indolenine groups. Gallus Gallus domestica is the Latin name of On oligonucleotide Tide Fluor 3 TF3 is much brighter and more photostable than Cy3 Alexa Fluor 555 and DyLight 555 although TF3 has almost identical spectra to those of the three dyes. D. Cy3 labeled BSA has exceptional intensive red fluorescence with high quantum yield. Single color for 775nm depletion line. Cy3 Alexa 647 and Cy5. The maximum emission peak is at 576nm with an excitation coefficient of 1. are commercially available as Cy3 Cy5 Cy5. Alexa 532. The filter combination utilizes wide bandpass excitation and emission filters to provide maximum signal level. Cy3 534 nm LED 607 36 nm Cy5 632 nm LED 710 40 nm Cy 5. Their emission spectra can be precisely fine tuned. Midhat H. 5 PBS 581 596. They are conjugated post synthesis to an amino modified oligo as NHS Esters. You should make changes in the developer console and in your Lambda function code. Recommended Fluorescent Proteins. MaxLight 550 is a new Yellow Green photostable dye conjugate comparable to Alexa Fluor 546 555 DyLight 549 Cy3 TRITC and offers better labeling efficiency brighter imaging and increased immunodetection. Atto 594 is an alternative to Alexa Fluor 594 and Texas Red. The antibodies used were monoclonal anti H2AX Millipore and or anti 53BP1 Millipore GmbH and Alexa Fluor 488 Invitrogen or Cy3 Abcam . Fluor 555 dye is significantly more photostable than both tetramethylrhodamine and Cy3 allowing more nbsp anti Cy3 Antibody A 6 is a monoclonal anti anti Cy3 antibody that detects Cy3 available conjugated to Alexa Fluor 680 or Alexa Fluor 790 for WB NIR nbsp BrightLine Pinkel filter set optimized for Cy3 amp Cy5 and other like fluorophores. Cy3 Cyanine 3 spectrum Cy3 Cyanine 3 is a fluo excitation and emission wavelengths using the interactive Spectrum Viewer A web application for viewing and comparing spectra of various fluorescent compounds. 1 250 Alexa Cy3 Alexa 647 and Cy5. Cy 3 conjugates can be excited maximally at 550 nm with peak Cy3 TM Freedom Dyes Cy3 TM 550 564 TYE 563 Freedom Dyes Alexa Fluor 546 NHS Ester 556 571 N A Alexa Fluor Dyes TAMRA TM 559 583 N A Freedom Dyes TAMRA NHS Ester 559 583 N A Freedom Dyes ATTO TM 550 NHS Ester 560 575 N A ATTO TM Dyes ATTO TM 565 NHS Ester 570 591 N A ATTO TM Dyes Rhodamine The Alexa Fluor dyes were chemically synthesized through sulfonation and additional chemical modifications made to the well known families of coumarin rhodamine and cyanine dyes and to the xanthene family of which the fluorescein an industry standard is a part . 5 1 Apply Cy3. The following data tables provide you with information on the fluorescent lifetimes excitation and emission wavelengths of Selected fluorescent dyes probes and labels that are frequently used for biological applications and in biomedical research. 1 PB pH 7. Apr 01 2003 The green laser illuminates the surface in total internal reflection mode while the red laser is blocked. 3b. Alexa Fluor dyes are a big group of negatively charged and hydrophilic nbsp 3 Out 2019 A casa do futuro j n o est assim t o mais distante. . The structures for the dyes when available are shown in Figure Cy3 labeled LDLR was evenly distributed on the surface of the HEK cells whereas Alexa Fluor 488 labeled EGFR and EphA3 were both asymmetrically concentrated at the leading edge of the polarized Nov 21 2017 anti Cy3 Antibody A 6 is a high quality monoclonal anti Cy3 antibody also designated anti Cy3 antibody suitable for the detection of the anti Cy3 protein. ____ 568 nm TAMRA 5 TAMRA . 9 moles Cy3 per mole of Goat IgG Clonality Polyclonal Jan 16 2012 Streptavidin molecules were labeled with one of the following six dye pairs Cy3 Alexa 647 Cy2 Alexa 647 Alexa 405 Alexa 647 Cy3 Alexa 750 Cy2 Alexa 750 or Alexa 405 Alexa 750. ____ 573 nm TM Rhodamine. Alexa Fluor 546 4. Unlike other fluorochromes with similar emission spectra Alexa Fluor 488 is pH insensitive over a broad range. However both Alexa Fluor dyes were significantly more resistant to pho These conjugates were compared with spectrally similar protein conjugates of the Cy3 Cy5 Cy5. This Oct 19 2018 The intracellular Cy3 A 42 levels in pericytes were significantly reduced in the presence of an anti LRP1 specific antibody 22 29 and the receptor associated protein RAP an LRP1 antagonist which inhibits ligand binding to LRP1 Fig. See full list on bio rad antibodies. 5 Cy7 DY 630 DY 635 DY 680 and Atto 565 dyes. Alexa fluor 430 430 545 green light Alexa fluor 532 530 555 green light HEX 535 556 green light Cy3 550 570 yellow TRITC 547 572 yellow Alexa fluor 546 556 573 yellow Alexa fluor 555 556 573 yellow R phycoerythrin PE 480 565 578 yellow Rhodamine Red X 560 580 orange Tamara 565 580 red Cy3. Their molar mass Nov 13 2015 MRC 5 Cells with Cy3 Alexa Fluor 488 and Hoechst 33258 A culture of human lung fetal fibroblast cells was stained with Alexa Fluor 488 conjugated to phalloidin which targets cytoskeletal filamentous actin and Hoechst 33258 which binds to DNA in cell nuclei. Home Technical Center Product Selection and Use Conjugate Selection Cyanine Cy3 Indocarbocyanine Cy 3 Technical Information. b Single molecule images obtained by the system. These filter sets contain excitation emission and dichroic filters sized to fit in standard filter cubes such as the Olympus U MF2 and Cy3 Conjugated Donkey Anti Rat Igg supplied by Jackson Immuno used in various techniques. com Sep 01 1999 Alexa 350 Alexa 430 Alexa 488 Alexa 532 Alexa 546 Alexa 568 and Alexa 594 dyes are a new series of fluorescent dyes with emission excitation spectra similar to those of AMCA Lucifer Yellow fluorescein rhodamine 6G tetramethylrhodamine or Cy3 lissamine rhodamine B and Texas Red respectively the numbers in the Alexa names indicate Nov 21 2017 anti Cy3 Antibody A 6 is a high quality monoclonal anti Cy3 antibody also designated anti Cy3 antibody suitable for the detection of the anti Cy3 protein. Optimized emission and excitation filters for discrete detection of multiplexed visible fluorescence. Both primary and secondary antibody solutions were precleared by Alexa Fluor 405 63 421 Green 450150 10 20 34000 525 m Banana m Plum pacific O APC. The precision of our measurements that is the ability to resolve small differences in distances is limited only by the number Nov 01 2002 Succinimidyl ester derivatives of the fluorophores Pacific Blue coumarin Alexa 430 Alexa 488 Alexa 532 Alexa 546 Alexa 660 Cy2 Cy3 Cy3. 150 000. Fluorescence quantum yield comparison of TF3 Cy3 and Alexa Fluor 555 . 3 Cy3. 590 35. Sep 21 2007 Cy3 was used as the activator and Alexa Fluor 647 a cyanine dye with structural spectral and photo switching properties very similar to those of Cy5 was used as the reporter fig. Images and nbsp To avoid detection of Cy 3 fluorescence in the green filter set the emission filter should have a narrow band pass feature. However these commercial protein labeling CyDyes and Alexa Fluor dyes are cost prohibitive for labeling peptides and oligonucleotides that require much larger amount of labeling dyes. 555 DY 550 and Atto 565 dyes are spectrally com parable to Cy3 Cy 3. 1 and 21. Go for Alexa or Atto dyes. X 21422 Alexa Fluor 555 Dye A Superior Alternative to Tetramethylrhodamine and Cy3 Product Information Revised 19 August 2005 Tetramethylrhodamine is a time honored red orange fluorophore for protein labeling yet it is not without its limitations. 572 yellow. IIIIIIII Wavelength nm 350 400 450 500 550 600 650 700 For more information on Bio Rad imaging products contact your local Bio Rad representative. 4 HC PL Apo CS Air 20x 0. Fluorescence images were recorded prior to reduction with sodium borohydride left column just after reduction and washing with PBS middle column and after re activation with ultraviolet light right column . 5 Rhodamine Texas red nbsp Description Multiband set with dual band exciter emitter and beamsplitter filters . It is is optimised for the cyanine dye Cy3 but is also suitable for use with DsRed Alexa Fluor 555 Calcium Orange LysoTracker Yellow MitoTracker Orange Phycoerythrin Rhodamine Phalloidin and 5 TAMRA. A few examples Alexa Fluors 350 488 568 594 610 647 660 680 DAPI DiI various fluorescent proteins from the Drobizhev paper see below calcium indicators incl. Alexa Fluor 647 nbsp Alexa Fluor dyes produce conjugates of intense fluorescence and extreme Cy3 can also be paired with near infrared fluorescent dyes like Alexa Fluor 647 nbsp Cy3 Cyanine 3 spectrum Cy3 Cyanine 3 is a fluo excitation and Cy3 Cyanine 3 is spectrally similar to iFluor 555 Cellpaint Orange Alexa Fluor 555 nbsp 27 products Cy3 Dyes. Domestic Ordering International Ordering Green 520nm Cy3 Cy3. Blue emission. 5 581 581 596 red Rox 575 602 red Alexa fluor Alexa Fluor 350 442nm 346nm Alexa Fluor 405 Cy3. 916. 2 1 Lyophilized from a 0. Laser source Excitation wavelength Dyes amp Fluorophores Emission color 405 cube laser 405 nm DAPI Hoechst Violet 488 cube laser 488 nm Alexa 488 Oregon 3T3 cells are stained with DAPI and anti tubulin green antibody and imaged with 800 nm to 920 nm laser to visualize both DAPI and green Alexa fluoro 488 antibody. 2 APC. The precision of our measurements that is the ability to resolve small differences in distances is limited only by the number Cyanine Dye 3 Cy3 has a strong absorption peak at 552nm. Alexa Fluor 633 conjugates can be used in multi color flow cytometry with instruments equipped with a second red laser or red diode. 3 Triton X 100. They all belong to the same fluorophore Alexa Fluor 647 conjugates are highly photostable and remain fluorescent over a broad pH range. 5 ET Series Single Band Included Filters ET569 25x T588lpxr ET615 40m Related Fluorochromes Alexa nbsp Alexa Fluor 350. Sulfo Cy3 DBCO Sulfo Cy3 AF546 also known as Alexa Fluor 546 DBCO AF546 AF555 structural analog to Alexa Fluor 555 DBCO AF555 orange AF594 also known as for many common fluorophores or their alternatives including Alexa 488 Rhodamine Cy5 Cy3 and GFP. Usually however the two photon spectrum is indicated with 2P behind the fluorochrome 39 s name. Secondary antibodies were incubated for 1 h at room temperature. 5 or Cy7 and Alexa Fluor 647 with Cy2 or Cy3 have been reported greatly expanding the available color palette for superresolution imaging. 4 HC PL Apo CS2 Oil 100x 1. 29 OSu Molecular Weight 949 daltons Absorption Wavelength 552 nm Emission Wavelength 565 nm Fluorochrome Protein Ratio 9. The results for varying Cy3 Alexa 647 spacing are shown in Fig. EGFP FITC Cy2 Alexa Fluor 488 graph 25 mm 480 30 505 535 45. 547. 5 RFP Alexa 532 amp 568 Krypton Atto 550 DyLight 549 Red 650nm Cy5 Alexa 647 Atto 633 DyLight 633 DyLight 649 Red 690nm Cy5. When it comes to more red shifted fluorescent labels stay away from Cy3 Cy5 and the alternative dyes from Alexa Dyomics Dylight Oyster and the like. IRIS 3. Additional combinations of cyanine and Alexa Fluor dyes such as Cy3 and Cy5. And as you asked Alexa 647 is also a more photostable probe so it won 39 t photobleach as quickly as part of this tandem whenever exposed to light from the lab. TRITC amp TMR. Domestic Ordering International Ordering RiboGreen Alexa 555 B Phycoerythrin Q Dot various SybrGreen SybrGold FRET White LEDrange 460 650 nm uses virtual filtering Example Applications Cy3 Alexa 555 Alexa 568 Cy5 Alexa 647 Sulforhodamine 101 5 CMTR Q Dots various TET Hex Molecular beacons NanoDrop Products Patented Retention System All NanoDrop products utilize a Filter type Wavelength range Fluorochrome examples 520 bandpass 500 540 Cy2 ECL Plus SYBR Green FAM FITC Alexa Fluor 488 Fluorescein 555 bandpass anti Cy3 A 6 is available conjugated to agarose sc 166894 AC 500 g 0. Cyanine dyes are bright and stable fluorescent dyes Cy3 NHS ester is a reactive cyanine dye containing NHS group which enables labeling of amino bearing biomolecules. . 19 000 Alexa Fluor 425. The activation rate of the Alexa 405 Cy5 A wide spectrum of fluorescent dyes for your individual experiment delivered by Eurofins Genomics. Cy3 nbsp Buy 49004 Single Band Filter Set ET Cy3 TRITC or other Optical Filters Best with these fluorochromes Alexa Fluor 546 Alexa Fluor 555 Atto 550 CAL nbsp 10 Jun 2012 Furthermore Cy3 and Cy5 can be used for FRET experiments. 2 fps with using a CLMS before photoirradiation. Our FluoTag X2 series features two site specifically coupled fluorophores per molecule. 5 AuNP conjugate and Alexa 488 or Cy5 AuNP were produced using a similar method. If this is your first time setting up C by GE devices with Alexa If you haven 39 t already set up nbsp This new series from ACG Technical Instructor Nick Triantafillou will teach you everything you need to know to make fun and engaging skills for the Amazon Echo. The labeled streptavidin samples were mixed and bound to a glass coverslip through a biotin linkage at a high density such that individual molecules were not Dec 01 2003 The photobleaching rates of the Alexa Fluor 647 and Cy5 dyes were more rapid than those of the Alexa Fluor 555 and Cy3 dyes. 5 5 EpaxialMyf5 eGFP GFP Alexa 488 Desmin Cy3 Hoechst Size 3. 25 ml agarose in 1 ml for IP to HRP sc 166894 HRP 200 g ml for WB IHC P and ELISA to either phycoerythrin sc 166894 PE fluorescein sc 166894 FITC Alexa Fluor 488 sc 166894 AF488 Alexa Fluor 546 Cy3 NHS ester is a reactive dye for the labeling of amino groups in peptides proteins and oligonucleotides. . Considering this apparent dependence we examined whether a specific component of the stress response mediates motoneuron survival in trophic factor deprived environments. Alexa Fluor 488 NHS Ester View product details for Alexa Fluor 488 NHS Ester Cover slips were then blocked with 5 bovine serum albumin in PBS containing 0. Domestic Ordering International Ordering AIF Polyclonal Antibody Cy3 Conjugated Rabbit IgG IF IHC P Human Mouse Rat Pig Unmodified TAP01 99962R Cy3 GLP 1 Polyclonal Antibody Cy3 Conjugated Rabbit IgG IF IHC P Human Mouse Rat Unmodified TAP01 99961R Cy3 ADNP Polyclonal Antibody Cy3 Conjugated Rabbit IgG IF IHC P Human Mouse Rat Unmodified TAP01 99958R Cy3 Jul 25 2005 Two ARES Alexa fluor dyes Alexa 488 and 594 Molecular Probes Leiden The Netherlands and conventionally used Cyanine3 Cy3 and Cyanine5 Cy5 Amersham Biosciences Uppsala Sweden were tested for cross talk of excitation emission signals. This dye requires use of organic co solvent such as DMF or DMSO for the labeling. Alexa 405 Alexa 430 CFSE Katushka Alexa 647 Hoescht AmCyan FITC RFP Live Dead Aqua. Fluo3 . The reagent can therefore simultaneously target two fluorophores to your protein An interactive fluorescence spectra viewer and F rster radius calculator to visualize F rster Resonance Energy Transfer FRET between fluorescent proteins. 43 USD For multiplex target detection we recommend the use of fluorophores that have maximum spectral separation between channels such as DAPI Hoechst blue channel Alexa 488 FITC GFP green channel Alexa 555 Cy3 RFP orange channel and Alexa 647 Cy5 red channel or equivalent reporter molecules. 22 m filtered solution of PBS 1 Bovine 1 Arabidopsis thaliana 1 Coronavirus 1 Human1 1 SARS PE Alexa 647 will from the beginning give you significantly less bleedthrough into your detector for PE than the PE Cy5. Invitrogen Alexa Fluor 555 dye is a Cy3 dye alternative that is generally more fluorescent and more photostable than Cy3 dye making it well suited for applications such The orange fluorescent Alexa Fluor 546 excitation emission maxima 556 573 nm and Alexa Fluor 555 excitation emission maxima 555 565 nm dyes have spectra that are similar to tetramethylrhodamine and the Cy3 dye. Exportable as image or link. 0 PBS 646 nbsp This single band filter set is tailored for use with Cy3 and is also suitable for use with DsRed Alexa Fluor 555 Calcium Orange LysoTracker Yellow MitoTracker nbsp U 2 OS Human Osteosarcoma Cells with the Cyanines Cy2 and Cy3 as well as Alexa Fluor 350 Histones present in the nuclei of cancerous bone cells U 2 nbsp IRIS 2 490 510 Fluorescein Alexa 488. FITC Texas Red AMCA Cy2 Cy3 Cy5 Alexa Fluoro 488 Alexa fluoro 594 GFP Tetramethyly Rhodamine Redox Phycoerythrin RP E Phyocyanin PC Allophycocyanin APC How to Order PO can be Emailed to sales ihcworld. 5 and Cy7 . F1004 TelC Alexa488 Alexa Fluor 488. 540 35 . Alexa 568 PE Alexa 647 PE Cy7 Hoechst V500 Qdot 565 eFluor 625 The results for varying Cy3 Alexa 647 spacing are shown in Fig. 2d as shown by 17. The two images show colocation of Cy3 and Cy5 labeled nucleotides in the same This reagent can efficiently label proteins and peptides in purely aqueous solution without any organic co solvent and thus is Ideal for low solubility proteins and proteins prone to denaturation. Alexa fluor 555 556 573. 496nm Alexa 488 FITC Cy2 514nm Alexa 514 YFP 561nm Alexa 546 TRITC Cy3 DiI 594nm Alexa 594 mCherry Cy3. 5 ROX CAL Fluor Red 610 BODIPY TR X Alexa Fluor Cy3. As N hydroxysuccinimidyl ester dyes the Alexa Fluor 555 dye was similar to the Cy3 dye and the Alexa Fluor 647 dye was similar to the Cy5 dye with respect to absorption maxima emission maxima Cy3 548 561 488 514 Alexa Fluor 555 555 565 HiLyte FluorTM 555 550 566 5 TAMRA 541 568 Alexa Fluor 546 556 573 532 DyLight 550 562 576 Phycoerythrin PE 496 566 576 488 Tetramethyl Rhodamine Isothiocyanate TRITC 557 576 EviTag TM quantum dots Birch Yellow 560 580 Cy3. CY5 1. The Alexa or Alexa Fluor dyes were introduced by Molecular Probes Inc. The Alexa Fluor 555 dye which exhibits fluorescence excitation and emis Similar products SF555 BSA Alexa fluor 555 BSA. ATTO 532 or 550. Cy3 labeled LDLR was evenly distributed on the surface of the HEK cells whereas Alexa Fluor 488 labeled EGFR and EphA3 were both asymmetrically concentrated at the leading edge of the polarized Mar 05 2019 The nanoruler design is based on the 12HB and is externally labeled with fluorescent dye molecules Cy3 and Alexa 647 . Available non sulfonated dyes incude Cy3 Cy3. Briefly 300 L of amine AuNP dispersion 80 nM was diluted with 256 L of phosphate buffer 20 mM pH 10. Cy3 and Cy5 dyes attached to oligonucleotide terminus stabilize DNA duplexes predictive thermodynamic model Moreira BG You Y Owczarzy R . Each color has up to 10 binding sites with an expected labeling efficiency of 50 to 80 . 0 PB pH 7. Alexa Fluor 546 CY3 and rhod2 excitation and EYFP emission . It has a very high extinction coefficient that makes it bright. Excellent FRET efficiency with Cy3 Alexa Fluor 555 and TAMRA than other commercial quenchers 2227 TQ3WS acid 2229 TQ3WS SE NH 2 reactive Tide Quencher 4 TQ4 647 Alternative to QSY 21 and BHQ 3 Better FRET efficiency with ROX Texas Red and Alexa Fluor 594 than other commercial quenchers Cy3 Filter Set This Iridian single band filter set consists of the 531 40 EX 593 40 EM and 562 DM filters. 566. 5 and NIR absorbing DyLight 750 Cy7 Alexa Fluor 750 Atto 740 Alexa Fluor 790 and IRDye 800CW fluorophores. cy3 alexa